picard对bwa生成的sam文件进行reorder时,报错如下:
Getting Help Exception in thread "main" htsjdk.samtools. SAM Format Exception: Error parsing text SAM file. MAPQ should be 0 for unmapped read.; File B2.sam; Line 18676 Line: ST-E00126:284:H37TJALXX:8:1101:4158:1872 101
解决办法:在命令行最后再加上 VALIDATION_STRINGENCY=LENIENT
可成功运行
时间: 2024-10-11 21:11:22